Mechanisms of generation of the rotational strengths in TEM-1 β-lactamase. Part II: theoretical study of the effects of the electrostatic interactions in the near-UV

The influences of the electrostatic environment on the mechanisms of generation of rotational strengths in near-UV of β-lactamase TEM-1 are studied in two aspects: the first is the effects of ionized tyrosine chromophores and the second – the effects of the changes of the excitation energies of the chromophores under the local electrostatic interactions. The mechanisms of generation of rotational strengths by tyrosinate chromophores are described. It is shown that both effects (tyrosine ionizations and Coulomb interactions) influence the mechanisms of rotational strengths and the relative contributions of the chromophores in the near-UV.