Fermentation and purification of cellulase from a novel strain Rhizopus stolonifer var. reflexus TP-02

The morphology, cultural characteristics and internal transcribed spacer’s (ITS)-5.8S rDNA analysis of TP-02 were applied to identify a strain of filamentous fungus Rhizopus TP-02 with high activity of cellulase. The result of that showed that the novel strain could be identified as Zygomycotina, Phycomycetes, Mucorales, Mucoraceae, Rhizopus stolonifer var. reflexus. The filter paper activity and CMC activity of the strain TP-02 could achieve 7.56 IU/mL and 28.5 IU/mL respectively after 96 h fermentation. During the tests of purification procedure, three types of Endo-β-1, 4-glucanases and two different molecular weight of β-1, 4-glucosidases were detected from the crud enzyme produced by the strain.