Author/Authors :
Pompa، نويسنده , , P.P. and Chiuri، نويسنده , , R. and Manna، نويسنده , , L. Penedo Pellegrino and A. Leipertz ، نويسنده , , T. and del Mercato، نويسنده , , L.L. and Parak، نويسنده , , W.J. and Calabi، نويسنده , , F. and Cingolani، نويسنده , , R. and Rinaldi، نويسنده , , R.، نويسنده ,
Abstract :
We show the possibility of realizing a hybrid system composed of a semiconductor nanoparticle (NP) and a metalloprotein, in which the photophysical properties of the two species can be exploited to elicit fluorescence resonance energy transfer mechanisms from the biomolecule to the NP. A specific conjugation process between CdSe/ZnS core/shell water soluble NPs, functionalized with surface exposed thiol groups (–SH), and the apo form of the metalloprotein azurin (Az) has been achieved, resulting in a fixed distance in the donor-acceptor pairs. The increase in the NP fluorescence intensity was found to be dependent on the Az to NP molar ratio.
