Author/Authors :
Tomo، نويسنده , , Tatsuya and Suzuki، نويسنده , , Takehiro and Hirano، نويسنده , , Emi and Tsuchiya، نويسنده , , Tohru and Miyashita، نويسنده , , Hideaki and Dohmae، نويسنده , , Naoshi and Mimuro، نويسنده , , Mamoru، نويسنده ,
Abstract :
A reversible spectroscopic change in chlorophyll (Chl) d has been demonstrated for the first time, using reduction with sodium dithionite and oxidation with potassium ferricyanide in an aqueous solution of detergent micelles. Chl d treated with a low concentration of NaBH4 in methanol showed the same results. The chemical structure of the reduced Chl d was determined by mass spectrometry to be 3-desvinyl-3-hydroxymethyl Chl a. These findings lead to a new hypothesis for the biosynthetic pathway of Chl d, being that Chl d is synthesized by oxidation of 3-desvinyl-3-hydroxymethyl Chl a, which is first formed enzymatically from Chl a.
