Title of article :
The effect of bovine serum albumin on batch and continuous enzymatic cellulose hydrolysis mixed by stirring or shaking
Author/Authors :
Shai and Brethauer، نويسنده , , Simone and Studer، نويسنده , , Michael H. and Yang، نويسنده , , Bin and Wyman، نويسنده , , Charles E.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Pages :
4
From page :
6295
To page :
6298
Abstract :
Bovine serum albumin (BSA) was applied as a model non-catalytic protein to enzymatic hydrolysis of Avicel and dilute acid pretreated corn stover at different reaction conditions to improve the understanding of its ability to enhance cellulose hydrolysis. Addition of BSA improved the 72 h hydrolysis yields in shake flasks by up to 26% for both substrates by reducing de-activation of the exoglucanases and by facilitating reductions in particle size and crystallinity during a magnetically stirred pre-incubation step. The enzyme stabilizing effect of BSA addition was most striking for batch hydrolysis in a stirred tank reactor, with glucose yields increasing by 76% after 72 h for Avicel and by 40% after 145 h for corn stover. Application of BSA to continuous hydrolysis for a mean residence time of 24 h gave 33% and 40% higher glucose yields for corn stover and Avicel compared to the controls.
Keywords :
BSA , Enzymatic hydrolysis , Continuous hydrolysis , cellulose , enzyme stability
Journal title :
Bioresource Technology
Serial Year :
2011
Journal title :
Bioresource Technology
Record number :
1924369
Link To Document :
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