Author/Authors :
Rze?nicka، نويسنده , , Izabela I. and Wurpel، نويسنده , , George W.H. and Bonn، نويسنده , , Mischa and van der Horst، نويسنده , , Michael A. and Hellingwerf، نويسنده , , Klaas J. and Matsunaga، نويسنده , , Soichiro and Yamada، نويسنده , , Taro and Kawai، نويسنده , , Maki، نويسنده ,
Abstract :
The adsorption of photoactive yellow protein (PYP) on a Au(1 1 1) surface and its fluorescence activity have been studied by electrochemical scanning tunneling microscopy (EC-STM) and fluorescence photometry. A stable, densely packed protein layer was observed after protein immobilization onto a Au(1 1 1) surface modified with a mixture of 3-mercaptopropanoic acid (3-MPA) and 11-mercaptoundecanoic acid (11-MUA) and subsequent formation of the amide bond with the use of N-hydroxysuccinimide and carbodiimide. Fluorescence photometry data indicate that covalent binding of PYP to the functionalized Au(1 1 1) surface does not interfere with the fluorescence properties of the native protein.
