Glucose oxidase biosensors based on carbon nanotube non-woven fabrics

Glucose oxidase biosensors were fabricated by vacuum-impregnation of a glucose oxidase in a polyvinyl alcohol (PVA) aqueous solution into carbon nanotube non-woven fabrics (CNTFs). The performance of the biosensor was evaluated by an electrochemical method, using the biosensors as working electrode, Pt wire as a counter electrode and Ag/AgCl as a reference electrode. The electrical conductivity of the CNTFs was tested with a four-probe method. Tensile strength was measured by a tensile tester. Results show that the response currents of the biosensors increase linearly with the glucose content in aqueous solutions. The linear range is from 2.5 to 30 mmol/L, the detection limit is 2.5 mmol/L and the response time is about 10 s. An acid treatment of the CNTF with 65% HNO3 can increase the response current.