Author/Authors :
Trantakis، نويسنده , , Ioannis A. and Fakis، نويسنده , , Mihalis and Tragoulias، نويسنده , , Sotirios S. and Christopoulos، نويسنده , , Theodore K. and Persephonis، نويسنده , , Peter and Giannetas، نويسنده , , Vassilis and Ioannou، نويسنده , , Penelope، نويسنده ,
Abstract :
The ultrafast dynamics of the DNA fluorescent dye Sybr Green I (SG) has been studied in buffer, single-stranded (ssDNA), double-stranded (dsDNA) and triple-stranded DNA (tsDNA). The fluorescence quantum yield of SG increases dramatically when bound to DNA (including tsDNA). The fluorescence dynamics of the free SG has shown two decay components with ∼0.15–0.4 ps and ∼1.3–2.1 ps time constants, depending on the fluorescence wavelength. Upon binding to DNA, the dynamics becomes slower exhibiting four decay components. This is mainly due to the restriction of the internal motions of the dye caused by the relatively rigid environment of the dye complexed with DNA.
