Author/Authors :
Silva، نويسنده , , Sônia M.L. and Coelho، نويسنده , , Letيcia N. and Malachias، نويسنده , , آngelo and Perez، نويسنده , , Carlos A. and Pesquero، نويسنده , , Jorge L. and Magalhمes-Paniago، نويسنده , , Rogério C. de Oliveira، نويسنده , , Mônica C.، نويسنده ,
Abstract :
The present study investigated the effect of the 6-monodeoxy-6-monoamine-β-cyclodextrin(Am-β-CD)/DNA (Am-β-CD/DNA) complex, as well as of culture medium components and proteins, at pH 7.4 and 5.0, on membranes of anionic and pH-sensitive liposomes comprised of DOPE–CHEMS, using energy dispersive X-ray diffraction (EDXD). At pH 7.4, the Am-β-CD/DNA complex induced the appearance of lamellar and hexagonal phases of DOPE. However, at pH 5.0, only non-lamellar phases could be observed. The presence of biological components led to a disruption of lipid order, but the pH-sensitivity of liposomes was maintained.
