Production of hydroxypyruvate from glycerate by a novel biotechnological route

In this work, bio-oxidation of glycerate was introduced for the green production of hydroxypyruvate. Whole cells of Pseudomonas sp. XP-LM were confirmed to have a good ability to produce hydroxypyruvate from glycerate. Under the optimal conditions, Hydroxypyruvate of 98.6 mM was produced from glycerate of 200 mM. Glycerate is now potentially producible from glycerol, a by-product during biodiesel fuel production, through a biotechnological process. Thus, the bioconversion system introduced in this work provided not only a green hydroxypyruvate production process but also a potential pathway of surplus glycerol utilization.