Title of article
High-level production of thermotolerant β-xylosidase of Aspergillus sp. BCC125 in Pichia pastoris: Characterization and its application in ethanol production
Author/Authors
Wongwisansri، نويسنده , , Sriwan and Promdonkoy، نويسنده , , Peerada and Matetaviparee، نويسنده , , Panida and Roongsawang، نويسنده , , Niran and Eurwilaichitr، نويسنده , , Lily and Tanapongpipat، نويسنده , , Sutipa Tanapongpipat، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2013
Pages
4
From page
410
To page
413
Abstract
A gene coding for thermotolerant β-xylosidase from Aspergillus sp. BCC125 was characterized. The recombinant enzyme was expressed in methylotrophic yeast Pichia pastoris KM71 and especially high yield of secreted enzyme was obtained. β-xylosidase possessed high enzyme efficiency (Kcat/Km = 198.8 mM−1 s−1) toward pNP-β-D-xylopyranoside (pNPβX) with optimal temperature and pH for activity of 60 °C and pH 4.0–5.0, respectively. The identified β-xylosidase showed clear synergism with previously identified xylanase for hydrolysis of xylan in vitro as well as simultaneous saccharification and fermentation process (SSF) in vivo with Pichia stipitis.
Keywords
?-xylosidase , Xylan hydrolysis , Pichia pastoris , Ethanol fermentation , Pichia stipitis
Journal title
Bioresource Technology
Serial Year
2013
Journal title
Bioresource Technology
Record number
1931793
Link To Document