Author/Authors :
Liang، نويسنده , , Liya and Liu، نويسنده , , Rongming and Li، نويسنده , , Feng and Wu، نويسنده , , Mingke and Chen، نويسنده , , Kequan and Ma، نويسنده , , Jiangfeng and Jiang، نويسنده , , Min and Wei، نويسنده , , Ping and Ouyang، نويسنده , , Pingkai، نويسنده ,
Abstract :
In this study, repetitive production of succinic acid from lignocellulose hydrolysates by enhancement of ATP supply in metabolically engineered E. coli is reported. Escherichia coli BA305, a pflB, ldhA, ppc, and ptsG deletion strain overexpressing ATP-forming phosphoenolpyruvate (PEP) carboxykinase (PEPCK), produced a final succinic acid concentration of 83 g L−1 with a high yield of 0.87 g g−1 total sugar in 36 h of three repetitive fermentations of sugarcane bagasse hydrolysate. Furthermore, simultaneous consumption of glucose and xylose was achieved, and the specific productivity and yield of succinic acid were almost maintained constant during the repetitive fermentations.
Keywords :
Repetitive production of succinic acid , Lignocellulose hydrolysates , Escherichia coli BA305 , ATP
