Title of article :
Butanol production by immobilised Clostridium acetobutylicum in repeated batch, fed-batch, and continuous modes of fermentation
Author/Authors :
Dolej?، نويسنده , , Igor and Kras?an، نويسنده , , Vladim?r and Stloukal، نويسنده , , Radek and Rosenberg، نويسنده , , Michal and Rebro?، نويسنده , , Martin، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
Abstract :
Clostridium acetobutylicum immobilised in polyvinylalcohol, lens-shaped hydrogel capsules (LentiKats®) was studied for production of butanol and other products of acetone–butanol–ethanol fermentation. After optimising the immobilisation protocol for anaerobic bacteria, continuous, repeated batch, and fed-batch fermentations in repeated batch mode were performed. Using glucose as a substrate, butanol productivity of 0.41 g/L/h and solvent productivity of 0.63 g/L/h were observed at a dilution rate of 0.05 h−1 during continuous fermentation with a concentrated substrate (60 g/L). Through the process of repeated batch fermentation, the duration of fermentation was reduced from 27.8 h (free-cell fermentation) to 3.3 h (immobilised cells) with a solvent productivity of 0.77 g/L/h (butanol 0.57 g/L/h). The highest butanol and solvent productivities of 1.21 and 1.91 g/L/h were observed during fed-batch fermentation operated in repeated batch mode with yields of butanol (0.15 g/g) and solvents (0.24 g/g), respectively, produced per gram of glucose.
Keywords :
Butanol , LentiKats® , Fermentation , immobilisation , Clostridium acetobutylicum
Journal title :
Bioresource Technology
Journal title :
Bioresource Technology