Organic matter characterization in a tropical estuarine-mangrove ecosystem of India: Preliminary assessment by using stable isotopes and lignin phenols

In order to characterize the sources and fate of organic matter (OM) in the Pichavaram estuarine-mangrove ecosystem (east coast of India), stable isotope (δ13C and δ15N) ratios and molecular lignin analyses were conducted in plant litter, benthic algae, sediment, particulate matter and in a variety of benthic invertebrate species. The δ13C signature of plant litter ranges from −29.75‰ to −27.64‰ suggesting that mangrove trees follow the C3 photosynthetic pathway. Sedimentary δ13C signature (−28.92‰ to −25.34‰) demonstrates the greater influence of plant litter organic matter on sedimentary organic matter. Suspended particulate organic pool was influenced by terrestrial source and also seems to be influenced by the marine phytoplankton. Enriched signature of δ15N in surface sediments (4.66–8.01‰; avg. 6.69‰) suggesting the influence of anthropogenic nitrogen from agricultural fields and human settlements. Spatial chemical variability in availability of nitrogen and plant associated microbial interactions demonstrate variability in δ15N signature in mangrove plant litter. Two (lower and higher) trophic levels of invertebrates were identified with and observed >4‰ gradient in δ13C signal between these two trophic groups. The observed δ13C values suggest that the lower level invertebrates feed on phytoplankton and higher level organisms have a mixed source of diet, phytoplankton, sediment and particulate organic matter. Lignin phenol analyses explain that the benthic surface layer was almost free of lignin. The ratio between syringyl phenols to vanillyl phenols (S/V) is 1.14–1.32 (avg. 1.23) and cinnamyl phenols to vanillyl phenols (C/V) is 0.17–0.31 (avg. 0.24), demonstrate non-woody angiosperm tissues was the major sources of lignin to this ecosystem, while aldehyde to acid ratios (Ad/Al) describe diagenetic nature of sediment and is moderately to less degraded. A two-end-member mixing model indicate that the terrigenous OM was dominant in the estuarine zones, while in the mangrove zone terrigenous supply accounts for 60% and marine input accounts for 40%.