Simultaneous determination of maltose and glucose using a dual-electrode flow injection system

A dual-electrode system consisting of a glucose oxidase (GOD) electrode and an amyloglucosidase/glucose oxidase (AG) electrode in an FIA manifold was constructed to determine maltose and glucose simultaneously in their mixture. Highly active enzyme membranes were acquired. The linear range of the system was 0·3–30 mmollitre of sugars. The variation coefficients were 3.2% for the GOD electrode and 1.6% for the AG electrode in measuring the mixture of glucose and maltose. The results made by the dual-electrode system agreed well with those made by Fehling titration. During 20 daysʹ determination, the activities of the enzyme electrodes showed no decay.