Generation of anti-zearalenone scFv and its incorporation into surface plasmon resonance-based assay for the detection of zearalenone in sorghum

Recombinant scFvs to zearalenone were isolated by phage display, expressed in Escherichia coli and the analytical sensitivity of selected scFvs determined by competitive inhibition enzyme-linked immunoassay (CI-ELISA) and surface plasmon resonance (SPR)-based inhibition assay. The results demonstrated higher sensitivity for the SPR-based assay compared to CI-ELISA. The applicability of the SPR-based assay for the detection of zearalenone in food samples was evaluated using sorghum.