Author/Authors :
Mizubuti، نويسنده , , Ivone Yurika and Biondo Jْnior، نويسنده , , Oswaldo and de Oliveira Souza، نويسنده , , Luiz Waldemar and dos Santos Ferreira da Silva، نويسنده , , Rui Sérgio and Ida، نويسنده , , Elza Iouko and Shimokomaki، نويسنده ,
Abstract :
Optimisation for pigeon pea protein extraction (Y) (Cajanus cajan (L) Millsp from the new IAPAR 43-Aratã variety) was investigated using response surface methodology. A compound central design was used with variables (X1) NaCl concentration (0.000; 0.025; 0.050; 0.075 and 0.100M); (X2) pH (2.5; 4.0; 5.5; 7.0 and 8.5) and (X3) liquid:solid ratio (5:1; 10:1; 15:1; 20:1; and 25:1, v/w). A model of the second degree equation was used to create the surface responses and confirmative studies were carried out. The following equation: Ŷ=−19.3733+8.6004x2−0.508526x22 shows optimum conditions for protein extraction of about 75% yield, at pH 8.5 without NaCl regardless of the liquid:solid ratio (v/w) under the experimental conditions studied.
