Determination of phenolic constituents in citrus juices: Method of high performance liquid chromatography

Some flavonoid compounds, naringin, hesperidin, neohesperidin, and quercetin, generally present in fruit juices were separated and determined by high performance liquid chromatography using a C6-phenyl-phase column and diode array detector. The method was partly validated with good results. Flavanones naringin, hesperidin, and neohesperidin were identified as markers of 100% Citrus juices. Their contents in analysed juices were compared with published values established by extensive research of Citrus composition. Quercetin, as a constituent of grapefruit and some other fruit juices, was also studied.