Purification of rosmarinic acid extracts from Lavandula vera MM cell biomass

Two approaches for purification of rosmarinic acid extracts, obtained from Lavandula vera MM cell biomass were investigated: adsorption on different Amberlite XAD resins and extraction with ethylacetate. Results showed that when ethylacetate extraction was used, 18.7 times higher level of purity compared to initial material was achieved. Similarly the obtained preparations from adsorption on Amberlite XAD-7 resin and elution with 80% ethanol contained 29.8% rosmarinic acid, which is 7.7 times higher purity compared to initial material. The precipitation of rosmarinic acid as Ca-rosmarinate was investigated as well. The obtained rosmarinic acid preparations were with low yield (34% of rosmarinic acid fell into sediment).