Cadmium-binding protein components of flaxseed: Influence of cultivar and location

The distribution of cadmium-(Cd)-binding components from five flaxseed cultivars grown at three locations in southern Manitoba was investigated to examine genotypic and environmental effects. Three protein fractions with different electrostatic properties, eluting at 0.10, 0.25 and 0.50 M NaCl by ion-exchange chromatography DEAE-Sephacel, represented 12%, 66% and 7% of the bound (extracted) protein, respectively, while 15% of the protein remained unbound. Cadmium and other divalent metal (zinc, copper and calcium) contents of protein fractions were strongly influenced by location. Cultivar differences in protein and cadmium contents of the protein fractions were highly significant. Cadmium and zinc accumulated similarly in the 0.10 and 0.25 M protein fractions at 51% and 40–43%, respectively. Transfer of copper occurred prominently in the 0.50 M fraction while most of the calcium (55%) remained unbound. The distribution of cadmium, zinc, copper and calcium in fractions of flaxseed proteins was strongly influenced by cultivar and location, indicating differences in their accumulation, migration and transfer.