Yellow discoloration of the liposome system of cuttlefish (Sepia pharaonis) as influenced by lipid oxidation

Lipid oxidation, discoloration, loss of amine groups and pyrrolization of the liposome systems of cuttlefish (Sepia pharaonis) in the presence of FeCl3 and ascorbic acid were studied. Thiobarbituric acid-reactive substances (TBARS) and the b∗-value of cuttlefish liposomes increased with a coincidental decrease in amine groups when the incubation temperatures (0, 4, 25, and 37 °C) and incubation times (0–24 h) were increased (p < 0.05). As lipid oxidation and yellow pigment formation in the cuttlefish liposome proceeded, a loss of amine groups and pyrrolization were also detected. The effects of FeCl3 and ascorbic acid, at different concentrations, on TBARS production, b∗-value, loss of amine groups and pyrrolization of cuttlefish liposome were also investigated. Both FeCl3 and ascorbic acid showed prooxidative effects in cuttlefish liposome in a concentration-dependent manner. Sodium chloride (0–2%) reduced TBARS, b∗-values and pyrrole compounds. These results suggest a positive correlation between lipid oxidation and the development of yellow pigments in cuttlefish phospholipids.