Measurement of caffeine in coffee beans with UV/vis spectrometer

In this research work using UV/vis spectrophotometer the molar decadic absorption coefficients and transitional dipole moment of pure caffeine in water and dichloromethane were obtained at 272 and 274.7 nm. The molar decadic absorption coefficients of caffeine in water and dichloromethane at these wavelengths are 1115 and 1010 m2 mol−1, respectively. The calculated values for the transitional dipole moment of caffeine in water and in dichloromethane are 10.40 × 10−30 and 10.80 × 10−30 C m, respectively. After characterizing caffeine in water and dichloromethane, fast and simple methods were developed that enable to quantify the content of caffeine in coffee beans. The methods helped in extracting caffeine from coffee dissolved in water by dichloromethane, and Gaussian fit was applied to eliminate the possible interference with the caffeine spectra.