Author/Authors :
de Souza Jْnior، نويسنده , , Waldeck Campanha and de Rezende، نويسنده , , Sebastiمo Tavares and Viana، نويسنده , , Pollyanna Amaral and Falkoski، نويسنده , , Daniel Luciano and Reis، نويسنده , , Angélica Pataro and Machado، نويسنده , , Solimar Gonçalves and Barros، نويسنده , , Everaldo Gonçalves de and Guimarمes، نويسنده , , Valéria Monteze، نويسنده ,
Abstract :
Whole cells of Debaryomyces hansenii UFV-1 were permeabilised with ethanol and immobilised in calcium alginate hydrogel. The optimum pH and temperature for α-galactosidase activities of permeabilised free (PFC) and permeabilised immobilised cells (PIC) were 4.5 and 60 °C; and 4.0 and 70 °C, respectively. PIC α-galactosidase was more stable than that of PFC. The incubation of PIC at 60 and 70 °C promoted an increase in α-galactosidase activity. The α-galactosidase activity was maintained when PIC was used in three repeated batches. The Km values for PIC and PFC α-galactosidases, with ρNPαGal, were 0.82 mM and 0.30 mM, respectively. Soy milk treatment with PIC for 6 h at 60 °C promoted 100% hydrolysis of raffinose oligosaccharides.
Keywords :
UFV-1 , ?-Galactosidase , Debaryomyces hansenii , Calcium Alginate , Cell immobilization , Galactooligosaccharides
