Sweet and bitter constituents of Wilbrandia species

Two novel cucurbitane glycosides, wilbrandisides A and B were isolated as sweet-taste substances from the root of Wilbrandia species (Cucurbitaceae) along with seven known cucurbitane glycosides. Their structures were determined by spectroscopic means, including two-dimensional NMR experiments. Their sweet-taste properties were evaluated by a human sensory panel test. Consequently, wilbrandiside A was shown to be 28 times sweeter than sucrose and was the compound having the most potent sweet taste of all the cucurbitane glycosides isolated from this plant.