Development of a class-specific monoclonal antibody-based ELISA for aflatoxins in peanut

Three class-specific monoclonal antibodies against aflatoxins were screened by a designed strategy in which aflatoxin G2 was used as competitor in the screening ELISA system. With a high cross-reactivity (65%) to aflatoxin G2, antibody 10C9 had the most similar sensitivity for five aflatoxins (AFB1, AFB2, AFG1, AFG2 and AFM1), whose I50 values were in a range of 2.1–3.2 ng ml−1. So, antibody 10C9 was selected to develop an ELISA for determination of aflatoxin B1, B2, G1, G2 and total of them in peanut samples. And spiked recoveries were from 87.5% to 102.0%. The results indicate that the ELISA developed can accurately determine total aflatoxins in samples of peanuts after the simple and rapid extraction procedure.