Analysis of trans fatty acids in deep frying oils by three different approaches

Simulated frying experiments were performed on three different types of oils with French fries as the fried food. Comparison of frying oil samples was then made with their control counterparts (i.e. oil samples heated without food). Three different methods, gas chromatography (GC), attenuated total reflection (ATR) AOCS method Cd 14d-99 and attenuated total reflection negative second derivative absorbance (−2D ATR), were applied to quantify total trans fats. The total trans fats were found to be higher in the frying oil samples as compared to the control samples, which might be due to the presence of a high amount of trans fats in the pre-fried and frozen French fries. In general, the ATR AOCS method Cd 14d-99 produced lower amounts of trans fatty acids and the −2D ATR absorbance method produced higher amounts when compared with those obtained by gas chromatography.