Evaluation of antioxidant and anti-initiating activities of crude polyphenolic extracts from seedless and seeded Indian grapes

The extracts of crude polyphenols (seeds, pulp + skin, whole) from four different cultivars of Indian grapes were used in this study. The total polyphenolic contents of grape polyphenolic extracts (GPEs) were determined and their in vitro antioxidant and anti-initiating activities evaluated. The total polyphenolic contents, expressed in terms of gallic acid/catechin/procyanidin B3 equivalents, were found to vary significantly. Antioxidant activity of GPEs, particularly the seedless variety, was evident from significant dose-dependent inhibition of lipid peroxidation and DPPH activity. GPEs and catechin inhibited the microsome-catalysed activity of cytochrome P450 isozymes (1A1, 1A2, 2B1) in a dose-dependent manner, by the decreased formation of resorufin. The inhibitory activity of GPEs on nitrite-mediated N-nitrosation of dimethylamine and N-methylaniline appears to correlate significantly with the total polyphenolic contents. Furthermore, six individual polyphenols present in GPEs were quantitated by HPLC, wherein procyanidin B3 was a major constituent.