Title of article :
Achillea millefolium L. s.l. herb extract: Antioxidant activity and effect on the rat heart mitochondrial functions
Author/Authors :
S. Trumbeckaite، نويسنده , , Sonata and Benetis، نويسنده , , Raimondas and Bumblauskiene، نويسنده , , Lina and Burdulis، نويسنده , , Deividas and Janulis، نويسنده , , Valdimaras and Toleikis، نويسنده , , Adolfas and Vi?kelis، نويسنده , , Pranas and Jak?tas، نويسنده , , Valdas، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Pages :
9
From page :
1540
To page :
1548
Abstract :
An extract of Achillea millefolium herb (YE) was investigated for antioxidant activity using chemical and biological assays. Qualitative and quantitative analysis of some major phenolics was carried out by HPLC. An on-line HPLC-DPPH assay showed that YE possesses significant antiradical activity which is due to the presence of active components amongst phenolic compounds. Furthermore, direct effects of YE and a mixture of its identified phenolic compounds (MPC) on isolated rat heart mitochondrial function were investigated. We found that YE in concentration-dependent manner induce a decrease in State 3 respiration rate without any changes in the integrity of inner mitochondrial membrane. Thus, pyruvate oxidation was affected only by the highest used YE concentrations; meanwhile succinate oxidation was reduced even at lower YE concentrations. MPC had no effect on mitochondrial State 3 respiration rate. Fluorimetric measurements demonstrated that YE at concentrations that had no effect on the State 3 respiration rate significantly decreased H2O2 production in mitochondria.
Keywords :
Yarrow , Achillea millefolium , Phenolic compounds , Mitochondria , Flavonoids , antioxidant
Journal title :
Food Chemistry
Serial Year :
2011
Journal title :
Food Chemistry
Record number :
1965332
Link To Document :
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