Author/Authors :
S. Trumbeckaite، نويسنده , , Sonata and Benetis، نويسنده , , Raimondas and Bumblauskiene، نويسنده , , Lina and Burdulis، نويسنده , , Deividas and Janulis، نويسنده , , Valdimaras and Toleikis، نويسنده , , Adolfas and Vi?kelis، نويسنده , , Pranas and Jak?tas، نويسنده , , Valdas، نويسنده ,
Abstract :
An extract of Achillea millefolium herb (YE) was investigated for antioxidant activity using chemical and biological assays. Qualitative and quantitative analysis of some major phenolics was carried out by HPLC. An on-line HPLC-DPPH assay showed that YE possesses significant antiradical activity which is due to the presence of active components amongst phenolic compounds. Furthermore, direct effects of YE and a mixture of its identified phenolic compounds (MPC) on isolated rat heart mitochondrial function were investigated. We found that YE in concentration-dependent manner induce a decrease in State 3 respiration rate without any changes in the integrity of inner mitochondrial membrane. Thus, pyruvate oxidation was affected only by the highest used YE concentrations; meanwhile succinate oxidation was reduced even at lower YE concentrations. MPC had no effect on mitochondrial State 3 respiration rate. Fluorimetric measurements demonstrated that YE at concentrations that had no effect on the State 3 respiration rate significantly decreased H2O2 production in mitochondria.
Keywords :
Yarrow , Achillea millefolium , Phenolic compounds , Mitochondria , Flavonoids , antioxidant