Title of article :
Vibrational and structural investigation of SOUL protein single crystals by using micro-Raman spectroscopy
Author/Authors :
Rossi، نويسنده , , Barbara and Giarola، نويسنده , , Marco and Mariotto، نويسنده , , Gino and Ambrosi، نويسنده , , Emmanuele and Monaco، نويسنده , , Hugo L.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
Pages :
5
From page :
87
To page :
91
Abstract :
Protein SOUL is a new member of the recently discovered putative heme-binding protein family called SOUL/HEBP and, to date, no structural information exists for this protein. Here, micro-Raman spectroscopy is used to study the vibrational properties of single crystals obtained from recombinant protein SOUL by means of two different optimization routes. This spectroscopic approach offers the valuable advantage of the in-situ collection of experimental data from protein crystals, placed onto a hanging-drop plate, under the same conditions used to grow the crystals. By focusing on the regions of amides I and III bands, some secondary structure characteristic features have been recognized. Moreover, some side-chain marker bands were observed in the Raman spectra of SOUL crystals and the unambiguous assignment of these peaks inferred by comparing the experimental Raman spectra of pure amino acids and their Raman intensities computed using quantum chemical calculations. Our comparative analysis allows to get a deeper understanding of the side-chain environments and of the interactions involving these specific amino acids in the two different SOUL crystals.
Keywords :
protein crystals , Protein SOUL , secondary structure , Raman spectroscopy , DFT computation
Journal title :
Journal of Molecular Structure
Serial Year :
2010
Journal title :
Journal of Molecular Structure
Record number :
1967471
Link To Document :
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