Author/Authors :
Taherian، Aliakbar نويسنده Obstetric and Gynecology Department, Isfahan University of Medical Sciences, Isfahan, Iran , , Mostafavi Zadeh، Seyed Mostafa نويسنده Graduate Student, Microbiology Department, Isfahan University of Medical Sciences, Isfahan, Iran , , Ghani، Hossein نويسنده Department of Internal Medicine, Kashan University of Medical Sciences, Kashan, Iran , , Khamechian، Tahere نويسنده Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran ,
Abstract :
Background: ST6Gal1, Cox-2 and HB-EGF genes are involved in different tumors
and their enhanced expressions often correlate with poor prognosis. In this study we
assay the expressions of these genes by reverse transcriptase-PCR in 54 breast cancer
samples.
Methods: Tissue samples were either formalin-fixed for histopathological
examination or frozen for reverse transcriptase-PCR. Image program was used for the
densitometry of the image of the gels and the expression of different genes was
normalized with beta actin expression. The studentʹs t-test and correlation matrix were
used for data analyses.
Results: We observed significantly higher expressions of ST6Gal1 (P= 0.040), Cox-
2 (P= 0.001) and HB-EGF (P= 0.009) in the tumor region compared to the margin
samples. A significant correlation was found between HB-EGF and Cox-2 expression
(P= 0.001). There was a positive correlation between total score, tumor size, histology
grade and nuclear grade but there was a reverse correlation between age and tumor size,
histology grade and total score.
Conclusion: Expressions of ST6Gal1, Cox-2 and HB-EGF in breast tumor samples
in this and a number of other studies emphasize their role as important markers in breast
cancer. The use of medications to inhibit either their individual expressions or the possible
inhibition of all three genes may improve patient survival and prevent metastasis.
