Simultaneous Spectrophotometric Determination of Amlodipine and Metoprolol by Principal Component Regression Multivariate Calibration and Comparison with HPLC

Multi-drug therapy in high blood pressure is appreciated to achieve a normal blood pressure. A simple spectrophotometric method based on principal component analysis has been proposed for simultaneous determination of amlodipine (Amd) and metoprolol (Met) in pharmaceutical preparations. Calibration matrix contains 3.402 µgml-1 to 170.130 µg ml-1 amlodipine and 13.7 µg ml-1 to 68.5 µgml-1 metoprolol. The proposed method was validated by using a set of synthetic sample mixtures and subsequently applied to simultaneous determination of amlodipine and metoprolol in different pharmaceutical preparations.