Title of article :
Construction of anticoagulant poly (lactic acid) films via surface covalent graft of heparin-carrying microcapsules
Author/Authors :
Li، نويسنده , , Juan and Zhu، نويسنده , , Baoqing and Shao، نويسنده , , Yingya and Liu، نويسنده , , Xinrong and Yang، نويسنده , , Xinlin and Yu، نويسنده , , Qun، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
Pages :
5
From page :
15
To page :
19
Abstract :
Heparin has been widely investigated in medicine and biomedical applications as an anticoagulant. This paper described a covalent modification of poly (lactic acid) (PLA) films with heparin-carrying microcapsules. The heparin-carrying microcapsules were achieved by layer-by-layer (LbL) self-assembly technology by which poly (allylamine hydrochloride) (PAH) and heparin were alternately coated on the Ca2+-cross-linked alginate hydrogel. The microcapsules were characterized by scanning electron microscopy (SEM), zeta potential analysis and Fourier transform infrared spectroscopy (FTIR), and then grafted to PLA films by –CONH– linkage between surface PLA molecules with residual primary amino groups on the outer PAH layer of microcapsules, in the presence of catalysts of 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). SEM and FTIR revealed the formation of –CONH– linkage. In vitro antithrombogenicity by the method of the activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) tests showed that surface-modified PLA films had superior coagulation properties to original PLA films. It is suggested that the present method would provide a potential effective tool for biomaterial modification.
Keywords :
HEPARIN , PLA films , microcapsules , Covalent engraftment , Antithrombogenicity
Journal title :
Colloids and Surfaces B Biointerfaces
Serial Year :
2009
Journal title :
Colloids and Surfaces B Biointerfaces
Record number :
1969869
Link To Document :
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