Author/Authors :
Wong، نويسنده , , Jack H. and Ip، نويسنده , , David C.W. and Ng، نويسنده , , T.B. and Chan، نويسنده , , Y.S. and Fang، نويسنده , , F. and Pan، نويسنده , , W.L.، نويسنده ,
Abstract :
A 5447 Da antifungal peptide with an N-terminal sequence highly homologous to plant defensins was purified from Phaseolus vulgaris cv. ‘King Pole Bean’ by anion-exchange chromatography on Q Sepharose and FPLC-gel filtration on Superdex 75. The isolated peptide inhibited growth of a number of fungal species, including Mycosphaerella arachidicola, Saccharomyces cerevisiae and Candida albicans, with IC50 values of 3.9, 4.0 and 8.4 μM, respectively. Using the membrane non-permeable DNA-binding dye SYTOX green, it was found that the peptide increased the cell membrane permeability of M. arachidicola, S. cerevisiae and C. albicans.
