Author/Authors :
Park، نويسنده , , Kwang-Il and Park، نويسنده , , Hyeon Soo and Nagappan، نويسنده , , Arulkumar and Hong، نويسنده , , Gyeong Eun and Lee، نويسنده , , Do Hoon and Kang، نويسنده , , Sang Rim and Kim، نويسنده , , Jin A. and Zhang، نويسنده , , Yung-Jue and Kim، نويسنده , , Eun Hee and Lee، نويسنده , , Won Sup and Shin، نويسنده , , Sung Chul and Hah، نويسنده , , Young-Sool and Kim، نويسنده , , Gon Sup، نويسنده ,
Abstract :
This study investigated the anti-proliferative and apoptotic effect of flavonoids isolated from Korean Citrus aurantium L. using A549 lung cancer cells. Flavonoids potently inhibited of A549 cells in a dose-dependent manner, whereas flavonoids had a weak inhibitory effect on proliferation of WI-38 cells. Flow cytometry and Western blot analysis showed that flavonoids induced cell cycle arrest at the G2/M checkpoint by controlling the proteins expression level of cyclin B1, cdc2, cdc25c and p21WAF1/CIP1. Also, flavonoids induced apoptosis through the regulation of the expression of caspases, cleaved PARP and Bax/Bcl-xL ratio. The activity of caspase-3 on A549 cells increased in a dose-dependent manner. These results clearly indicated that the anti-cancer effect of flavonoids on A549 cells follows multiple cellular pathways through G2/M arrest and the induction of apoptosis.
Keywords :
apoptosis , Human lung cancer cells , Korean Citrus aurantium L. , Cell cycle arrest , Flavonoid
