Secondary metabolites from the unripe pulp of Persea americana and their antimycobacterial activities

The fruits of Persea americana (Avocado) are nowadays used as healthy fruits in the world. Bioassay-guided fractionation of the active ethyl acetate soluble fraction has led to the isolation of five new fatty alcohol derivatives, avocadenols A–D (1–4) and avocadoin (5) from the unripe pulp of P. americana, along with 12 known compounds (6–17). These structures were elucidated by spectroscopic analysis. Among the isolates, avocadenol A (1), avocadenol B (2), (2R,4R)-1,2,4-trihydroxynonadecane (6), and (2R,4R)-1,2,4-trihydroxyheptadec-16-ene (7) showed antimycobacterial activity against Mycobacterium tuberculosis H37RV in vitro, with MIC values of 24.0, 33.8, 24.9, and 35.7 μg/ml, respectively.