A hydrogen peroxide biosensor based on the direct electron transfer of hemoglobin encapsulated in liquid-crystalline cubic phase on electrode

Liquid crystal cubic phase formed with monoolein has been used as immobilizing matrix to host redox protein hemoglobin on glassy carbon electrode surface. The promoted direct electron transfer between hemoglobin and electrode was observed and a large average kinetic electron transfer rate constant ks of 3.03(±0.02) s−1 was estimated. The electrode modified with cubic phase containing hemoglobin retains the bioactivity of hemoglobin and shows excellent bioelectrocatalytic activity to the reduction of hydrogen peroxide with a small apparent Michaelis–Menten constant of 0.25(±0.03) mM. A novel reagentless hydrogen peroxide biosensor was constructed using the hemoglobin-containing cubic phase modified electrode and the proposed hydrogen peroxide biosensor shows a linear range of 7.0–239 μM with a detection limit of 3.1(±0.2) μM and good stability and reproducibility.