Interaction of cytochrome c with zinc oxide nanoparticles

The influence of pH on the interaction between horse heart ferricytochrome c (cyt c) and zinc oxide nanoparticles (ZnO NPs) has been studied by a small angle scattering as well as UV–vis and FTIR spectroscopy. The observations showed that the optimal pH for the association of protein with nanoparticles is in pH range 5.0–8.0. no significant change in structure and thermodynamic stability of cytochrome c after the association with 60 nm ZnO NPs was performed by UV–vis and by a circular dichroism spectroscopy.