Title of article
A novel reusable PAni-PVA-Amylase film: Activity and analysis
Author/Authors
Singh، نويسنده , , Susmita and Saikia، نويسنده , , Jyoti Prasad and Buragohain، نويسنده , , Alak Kumar، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2013
Pages
5
From page
46
To page
50
Abstract
Alpha-amylase was immobilized onto polyaniline-polyvinyl alcohol (PAni-PVA) film by cross linking with glutaraldehyde. The activity yield and immobilization efficiency was calculated as 13.58% and 30.60% respectively. The PAni-PVA-Amylase films were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and amylase activity assay. The immobilized α-amylase had its optimum activity at pH 7.0 and 25 °C. The immobilization of α-amylase onto the PAni-PVA films protected the enzyme from Fe2+ inhibition. The PAni-PVA-Amylase films could be used effectively for 30 cycles with 60% retention of the activity.
Keywords
alpha-amylase , Immobilization , stability , Polyaniline
Journal title
Colloids and Surfaces B Biointerfaces
Serial Year
2013
Journal title
Colloids and Surfaces B Biointerfaces
Record number
1976349
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