Attenuation of histopathological alterations of colon, liver and lung by dietary fibre of barley Rihane in azoxymethane-treated rats

This study was carried out to determine the effect of dietary fibre (DF) of barley Rihane (BR) in the attenuation of colon, liver and lung histopathology alterations induced by azoxymethane (AOM) in rats. Rats were fed a control (C) or experimental diet containing 30% of BR. The intended rats for cancer treatment received two successive subcutaneous injections of azoxymethane (AOM) at 20 mg/kg body weight. The colons were analyzed for crypt multiplicity after 12 weeks of treatment. A histological study of the colon, liver and lungs was determined. The results showed that the BR diet significantly reduced the number of aberrant crypt per focus and altered their distribution. In addition, DF of BR increased significantly the mucus secretion compared to control group. The use of the AOM as colon specific carcinogen substance altered the liver and lung architectures, whereas the presence of DF of BR could be a protective factor for these organs.