Title of article :
Fabrication and characterization of a chemically oxidized-nanostructured porous silicon based biosensor implementing orienting protein A
Author/Authors :
J. and Naveas، نويسنده , , Nelson and Hernandez-Montelongo، نويسنده , , Jacobo and Pulido، نويسنده , , Ruth and Torres-Costa، نويسنده , , Vicente and Villanueva-Guerrero، نويسنده , , Raْl and Garcيa Ruiz، نويسنده , , Josefa Predestinaciَn and Manso-Silvلn، نويسنده , , Miguel، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
Pages :
7
From page :
310
To page :
316
Abstract :
Nanostructured porous silicon (PSi) elicits as a very attractive material for future biosensing systems due to its high surface area, biocompatibility and well-established fabrication methods. In order to engineer its performance as a biosensor transducer platform, the density of immunoglobulins properly immobilized and oriented onto the surface needs to be optimized. In this work we fabricated and characterized a novel biosensing system focusing on the improvement of the biofunctionalization cascade. The system consists on a chemically oxidized PSi platform derivatized with 3-aminopropyltriethoxysilane (APTS) that is coupled to Staphylococcus protein A (SpA). The chemical oxidation has previously demonstrated to enhance the biofunctionalization process and here “by implementing SpA” a molecularly oriented immunosensor is achieved. The biosensor system is characterized in terms of its chemical composition, wettability and optical reflectance. Finally, this system is successfully exploited to develop a biosensor for detecting asymmetric dimethylarginine (ADMA), an endogenous molecule involved in cardiovascular diseases. Therefore, this work is relevant from the point of view of design and optimization of the biomolecular immobilization cascade on PSi surfaces with the added value of contribution to the development of new assays for detecting ADMA with a view on prevention of cardiovascular diseases.
Keywords :
Biofunctionalization , Biosensing , Porous silicon , ADMA detection , protein immobilization , protein A
Journal title :
Colloids and Surfaces B Biointerfaces
Serial Year :
2014
Journal title :
Colloids and Surfaces B Biointerfaces
Record number :
1978091
Link To Document :
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