Title of article :
Effect of iron oxide loading on magnetoferritin structure in solution as revealed by SAXS and SANS
Author/Authors :
Meln?kov?، نويسنده , , L. and Petrenko، نويسنده , , V.I. and Avdeev، نويسنده , , M.V. and Garamus، نويسنده , , V.M. and Alm?sy، نويسنده , , L. and Ivankov، نويسنده , , O.I. and Bulavin، نويسنده , , L.A. and Mitr?ov?، نويسنده , , Z. and Kop?ansk?، نويسنده , , P.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2014
Pages :
7
From page :
82
To page :
88
Abstract :
Synthetic biological macromolecule of magnetoferritin containing an iron oxide core inside a protein shell (apoferritin) is prepared with different content of iron. Its structure in aqueous solution is analysed by small-angle synchrotron X-ray (SAXS) and neutron (SANS) scattering. The loading factor (LF) defined as the average number of iron atoms per protein is varied up to LF = 800. With an increase of the LF, the scattering curves exhibit a relative increase in the total scattered intensity, a partial smearing and a shift of the match point in the SANS contrast variation data. The analysis shows an increase in the polydispersity of the proteins and a corresponding effective increase in the relative content of magnetic material against the protein moiety of the shell with the LF growth. At LFs above ∼150, the apoferritin shell undergoes structural changes, which is strongly indicative of the fact that the shell stability is affected by iron oxide presence.
Keywords :
contrast variation , apoferritin , Small-angle neutron scattering , Small-Angle X-Ray Scattering , Magnetoferritin , Biorelevant magnetic nanoparticles
Journal title :
Colloids and Surfaces B Biointerfaces
Serial Year :
2014
Journal title :
Colloids and Surfaces B Biointerfaces
Record number :
1979039
Link To Document :
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