Application of FTIR-ATR spectroscopy to the quantification of sugar in honey

A Fourier transform infrared spectroscopic method with attenuated total reflectance (FTIR-ATR) and partial least squares (PLS) regression model for the prediction of sugar content in honey samples was calculated. rds of trehalose, glucose, fructose, sucrose, melezitose, turanose and maltose were used to identify and quantify the individual sugar components in 63 honey samples by HPAEC-IPAD. se and glucose are the highest sugars in honey with an average value of 36% and 26%, respectively. tDer spectra with MSC or SLS in the wave number range from 1500 to 750 cm−1 provide the best calibration model with a r2 of 86.60 and 86.01 with RPD of 2.6 and 2.55, respectively for fructose and glucose. ranose and melezitose good models were also found. IR-ATR showed to be a good methodology to quantify the main sugar content in honey and easily adapted to routine analysis.