Measurement and correlation of partition coefficients of hydrolytic enzymes for dextran+poly (ethylene glycol) + water aqueous two-phase systems at 20°C

The partition coefficients of hydrolytic enzymes, α-amylase, β-amylase and glucoamylase for the dextran (DEX)+poly(ethylene glycol) (PEG)+water aqueous two-phase systems with various polymer molecular weights were measured at 20°C. The partition coefficients obtained were correlated by using a mod fied Flory-Huggins equation which was empirically proposed for aqueous systems. The interaction parameters required were determined by fitting the model to the experimental data. The partition coefficients could be correlated with good agreement.