Author/Authors :
Kohiyama، نويسنده , , Cلssia Yumie and Yamamoto Ribeiro، نويسنده , , Milene Mayumi and Mossini، نويسنده , , Simone Aparecida Galerani and Bando، نويسنده , , Erika and Bomfim، نويسنده , , Natلlia da Silva and Nerilo، نويسنده , , Samuel Botiمo and Rocha، نويسنده , , Gustavo Henrique Oliveira and Grespan، نويسنده , , Renata and Mikcha، نويسنده , , Jane Martha Grat، نويسنده ,
Abstract :
The antifungal and antiaflatoxigenic properties of Thymus vulgaris essential oil (TEO) were evaluated upon Aspergillus flavus “in vitro”. Suspension containing 106 of A. flavus were cultivated with TEO in concentrations ranging from 50 to 500 μg/mL. TEO reached minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) at 250 μg/mL. Inhibition of ergosterol biosynthesis was detected at a concentration of 100 μg/mL of TEO. Morphological evaluation performed by both light microscopy and scanning electron microscopy showed that antifungal activity of TEO could be detected starting at a concentration of 50 μg/mL and the fungicide effect at a concentration of 250 μg/mL. TEO completely inhibited production of both B1 and B2 aflatoxins (AFB1 and AFB2) at a concentration of 150 μg/mL. This way, fungal biomass development and aflatoxin production were dependent on TEO concentration. Therefore, TEO was capable of controlling the growth of A. flavus and its production of aflatoxins.
Keywords :
Antifungal , Mycotoxins , Thyme essential oil , ergosterol , ASPERGILLUS FLAVUS
