Title of article :
Detecting quinoxaline-2-carboxylic acid in animal tissues by using sensitive rapid enzyme-linked immunosorbent assay and time-resolved fluoroimmunoassay
Author/Authors :
Le، نويسنده , , Tao and Yu، نويسنده , , Huan and Niu، نويسنده , , Xiaodong، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2015
Pages :
7
From page :
85
To page :
91
Abstract :
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and time-resolved fluoroimmunoassay (TR-FIA) based on an anti-N-butylquinoxaline-2-carboxamide (BQCA) monoclonal antibody were standardized and validated for quinoxaline-2-carboxylic acid (QCA) screening in animal tissues and its performance were compared to HPLC. The sensitivities obtained for edible tissue extracts were 1.62 and 1.12 ng ml−1 for ic-ELISA and TR-FIA detection, respectively. Two samples were spiked with QCA and analyzed by both methods. The recovery values ranged from 92.6% to 112.2% and the coefficients of variation were less than 15% for QCA spiking into swine tissue samples at concentrations of 2.5–50.0 μg kg−1. Excellent correlations (r2 = 0.987–0.996) of the ic-ELISA/HPLC and TR-FIA/HPLC data were observed for processed samples. The results demonstrated that the ic-ELISA and TR-FIA methods were rapid and accurate for the residue detection of QCA in animal tissues.
Keywords :
Carbadox , Quinoxaline-2-carboxylic acid , Indirect competitive enzyme-linked immunosorbent assay , time-resolved fluoroimmunoassay , monoclonal antibody , Animal tissue
Journal title :
Food Chemistry
Serial Year :
2015
Journal title :
Food Chemistry
Record number :
1980533
Link To Document :
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