Author/Authors :
Moravvej، Ali نويسنده Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences , , Jeddi Tehrani]، Mahmood نويسنده , , Salek Moghaddam، Ali Reza نويسنده Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences , , Dokouhaki، Pouneh نويسنده , , Shekarabi، Mahdi نويسنده Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences , , Ghods، Roya نويسنده Department of Immunochemistry, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Shahbazi، Mahdi نويسنده Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences , , Ghasemi، Jamileh نويسنده Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR , , Danesh، Parivash نويسنده Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences , , Mahmoudi، Ahmad Reza نويسنده Department of Immunochemistry, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Zarnani، Amir Hassan نويسنده Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IR Iran ,
Abstract :
Prevalence of abortion is higher in women with autoimmune thyroid disease. In the majority of cases, however, no abnormality of thyroid function is detected despite the high levels of antithyroid antibodies. The direct influence of such harmful autoantibodies in female reproductive organs may serve a role in pregnancy loss. In this study, expression of thyroglobulin in the reproductive tissues of cycling mice has been evaluated. Stages of estrous cycle were determined by cellular morphology and ratio of epithelial cells to leukocytes in vaginal smear of Balb/C mice. At each phase, the mice were sacrificed and their uterus, ovary and fallopian tubes were removed. Expression of thyroglobulin-specific transcript in endometrium was investigated by two sets of primers using reverse transcriptase-polymerase chain reaction (RT-PCR). In addition, expression of thyroglobulin in reproductive tissues was assessed by immunohistochemistry and dot blot analysis. The results showed that thyroglobulin mRNA is not expressed in endometrial tissue of Balb/C mice at any stage of estrous cycle. Immunohistochemical analysis also confirmed that thyroglobulin or its cross reactive-antigens are not expressed at the protein level in the female reproductive organs. The results showed that thyroglobulin was not expressed in the reproductive organs of female mice. It is plausible that antithyroglobulin antibodies could interact with newly-generated antigens during placentation and pregnancy.
