Author/Authors :
Hadavi، Reza نويسنده Department of Antibody-Antigen Engineering, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Zarnani، Amir Hassan نويسنده Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IR Iran , , Ahmadvand، Negah نويسنده Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR , , Mahmoudi، Ahmad Reza نويسنده Department of Immunochemistry, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Bayat، Ali Ahmad نويسنده Department of Immunochemistry, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Mahmoudian، Jafar نويسنده Department of Immunochemistry, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Sadeghi، Mohammad Reza نويسنده Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR , , Soltanghoraee، Haleh نويسنده Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR , , Akhondi، Mohammad-Mehdi نويسنده , , Tarahomi، Majid نويسنده Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran , , Jeddi Tehrani]، Mahmood نويسنده , , Rabbani، Hodjattallah نويسنده Immune and Gene Therapy Laboratory, CCK, Karolinska University Hospital Solna Department of Antigen and Antibody Engineering, Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR ,
Abstract :
We have employed a peptide-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N- or O-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. A wide variety of nestin proteins ranging from 140-250 kDa was detected by this antibody. This antibody is highly specific and functional in applications such as ELISA, flow cytometry, immunocytochemistry, and Western blot assays.
