Title of article
Differentiation of Bovine Spermatogonial Stem Cells into Osteoblasts
Author/Authors
Qasemi-Panahi، Babak نويسنده Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran. , , Tajik، Parviz نويسنده , , Movahedian، Mansooreh نويسنده Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University , , Moghaddam، Gholamali نويسنده Professors, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran , , Barzgar، Younes نويسنده Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz , , Heidari-Vala، Hamed نويسنده Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran ,
Issue Information
فصلنامه با شماره پیاپی 10 سال 2011
Pages
5
From page
149
To page
153
Abstract
Spermatogonial Stem Cell (SSC) technologies provide multiple opportunities for research in the field of biotechnology and regenerative medicine. The therapeutic use of Embryonic Stem Cells (ESCs) is restricted due to severe ethical and immunological concerns. Therefore, we need a new pluripotent cell type. Despite well-known role of germ cells in the gametogenesis, some facts apparently show their multipotentiality. In the present study, bovine SSCs were co-cultured with Sertoli cell for 7 days. Sertoli cells and SSCs were identified by Vimentin and Oct-4 immunocytochemical staining method, respectively. In order to differentiate SSCs into osteoblasts, we used consecutive inducer media without separation of the colonies. We characterized osteoblasts using Alizarin red staining.
Journal title
AJMB Avicenna Journal of Medical Biotechnology
Serial Year
2011
Journal title
AJMB Avicenna Journal of Medical Biotechnology
Record number
1982826
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