Title of article
Constructing a Mouse Oct4 Promoter/EGFP Vector, as a Whole-Cellular Reporter to Monitor the Pluripotent State of Cells
Author/Authors
Ghorbani، Reza نويسنده , , Emamzadeh، Abdolrahman نويسنده Department of Biology, Faculty of Sciences, University of Isfahan , , Khazaie، Yahya نويسنده , , Dormiani، Kianoush نويسنده Department of Molecular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran , , Ghaedi، Kamran نويسنده , , Rabbani، Sayed Mohammad Reza نويسنده , , Foruzanfar، Mahboubeh نويسنده Department of Molecular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran , , Karbalaie، Khadijeh نويسنده Department of Cell and Molecular Biology, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran , , Karamali، Fereshteh نويسنده Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR , , Lachinani، Liana نويسنده Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology , , Kiani-Esfahani، Abbas نويسنده Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR , , Nematollahi، Marzieh نويسنده , , NASR ESFAHANI، MOHAMMAD HOSSEIN نويسنده ,
Issue Information
فصلنامه با شماره پیاپی 16 سال 2013
Pages
8
From page
2
To page
9
Abstract
Background: The transcription factor Oct-4, is an important marker of undifferentiating level and a key regulating factor for maintenance of pluripotency in cells. Establishment of an Oct-4 promoter-based reporter system is an appropriate tool for monitoring the differentiation of embryonic stem cells both in vivo and in vitro.
Methods: In the present study, we report construction of a recombinant vector, pDB2 Oct4 promoter/EGFP, in which expression of Enhanced Green Fluorescent Protein (EGFP) was controlled by the mouse Oct-4 promoter.
Results: In transfected mouse embryonic stem cells with this vector, EGFP was predicted to be specifically expressed in pluripotency state. After transfection, high-level expression of EGFP under the control of Oct-4 promoter was observed in manipulated embryonic stem cells.
Conclusion: Thus, our new cellular reporter showed that both the properties of embryonic cells and expression the EGFP could be of great help in studying the differentiating and reprogramming mechanisms of mESCs.
Journal title
AJMB Avicenna Journal of Medical Biotechnology
Serial Year
2013
Journal title
AJMB Avicenna Journal of Medical Biotechnology
Record number
1983020
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