Author/Authors :
Kanungo، Satyajit نويسنده P.G. Department of Botany, Biochemistry and Molecular Biology Laboratory, Utkal University, Bhubaneswar-751004, Odisha, India , , Ranjan Rout، Jyoti نويسنده P.G. Department of Botany, Biochemistry and Molecular Biology Laboratory, Utkal University, Bhubaneswar-751004, Odisha, India , , Lata Sahoo، Santi نويسنده P.G. Department of Botany, Biochemistry and Molecular Biology Laboratory, Utkal University, Bhubaneswar-751004, Odisha, India ,
Abstract :
Background: Withania somnifera L. Dunal, (Ashwagandha) belongs to the family Solanaceae and is known as ‘Indian ginseng’ in the traditional system of medicine.
Objectives: In the present investigation, comparative analysis was carried out between two diffrent ecotypes of W. somnifera related tototal soluble protein and antioxidant enzyme activity [catalase (CAT), superoxide dismutase (SOD) and guaiacol peroxidase (GPX)] to fidout the expression of isozyme in response to climatic variation.
Materials and Methods: Viable and healthy seeds were germinated under the optimal condition to develop the in vitro and in vivo plants.
The plant materials were taken for the analysis of total soluble protein and antioxidant enzyme activity in both ecotypes.
Results: Maximum protein (15.54 mg/gm fresh weight) was quantifid from the in vitro grown plants of W1 ecotype. The CAT and SOD activity was found to be the maximum in W1 plants whereas, GPX activity was more in W2 plants. Specifi activity staining of isozyme pattern exhibited only one band of CAT in all types of plants. In case of SOD, four isoforms (SOD 1, SOD 2, SOD 3 and SOD 4) were observed in W1 ecotype whereas, one band (SOD 1) was missing in W2.
Conclusions: The supplementation of required nutrients under in vitro condition enhanced the yield of antioxidant enzymes. The geographical distribution of plants contributes to the variation in the expression of the isozyme in the same species, and that may be due to the climatic variation.
